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doi: 10.1111/j.1473-2165.2012.00613.x
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The homogenate was filtered through a 100-μM cell strainer and centrifuged at 1,000 g for 10 min at 4 °C, then the nuclear pellet was washed once in nucleus preparation buffer followed by resuspension in PBS with 1% BSA and 0.2 U µl Roche Protector RNase inhibitor
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